강의계획서
| 교과목코드 | JDE01205 | 교과목명 | 원핵생물실험1 |
|---|---|---|---|
| 강의학과 | 생명과학정보학과 | 교수 | 이상희 |
| 교수소속 | 생명과학정보학과 | 이수학년 | 2학년 |
| 과목구분 | 실습 | 과정구분 | |
| 이메일 | sangheelee@mju.ac.kr | 전화번호 | 031-330-6195 |
| 주차 | 주제 |
|---|---|
| 1주차 | Introduction and team grouping |
| 2주차 | Isolation of chromosomal DNA from bacteria |
| 3주차 | Polymerase chain reaction (PCR) |
| 4주차 | Preparation of competent cells |
| 5주차 | Agarose gel electrophoresis of PCR products |
| 6주차 | Gel extraction from agarose to isolate the PCR products |
| 7주차 | Ligation between PCR products and TA cloning vector |
| 8주차 | Transformation of ligation product into competent cell |
| 9주차 | Preparation of plasmid vector DNA from transformants |
| 10주차 | Agarose gel electrophoresis of plasmid vector DNA treated with restriction enzymes |
| 11주차 | Precipitation of DNA by ethanol |
| 12주차 | Transformation of recombinant DNA molecule into competent cell |
| 13주차 | Gene expression |
| 14주차 | Determination of expression product by SDS-PAGE |
| 15주차 | Final term examination |
| 16주차 |